Monday 7.13.2009
9:30-12 |
Activity 2-1: Alcian Blue Cartilage Stain
Activity 2-2: Alkaline Phosphatase Stain of Vessels
|
12-1 |
Lunch |
1-4 |
Continue Activities 2-1 & 2-2 & Imaging Image J Tutorial |
4-4:30 |
Crossing Zebrafish |
4:30-5 |
Lab Clean-up |
Tuesday 7.14.2009
9:30-10 |
Collect embryos & Clean plates |
10-11 |
Activity 2-3: PCR Reaction
|
11-12 |
Fluorescent microscopy |
12-1 |
Lunch |
1-3 |
Gel Electrophoresis |
3-4 |
Independent Experiment Planning |
4-4:30 |
Cross Fish |
4:30-5 |
Lab Clean-up |
Wednesday 7.15.2009
9:30-10 |
Collect embryos & Clean plates |
10-12 |
Activity 2-4: Acridine Orange Staining
Activity 2-3: PCR |
12-1 |
Lunch |
1-3 |
Phalloidin Information Sheet (Invitrogen) Gel Electrophoresis & Sequencing Prep |
3-4 |
Fix embryos for primary/secondary antibody staining |
4-5 |
Independent Experiment Planning & Lab clean-up |
Thursday 7.16.2009
9:30-12 |
Independent Experiment |
12-1 |
Lunch |
1-4 |
Alkaline Phosphatase Staining of 52hpf embryos, primary antibody |
4-4:30 |
"Lab Meeting" |
4:30-5 |
Lab Clean-up |
Friday 7.17.2009
9:30-10 |
Wash antibody, add secondary |
10-12 |
Independent Experiment/Georgette Charles, UCSF graduate student |
12- |
Lunch |
1-4 |
Wash secondary antibody, continue independnet experiment |
4-4:30 |
Cross Fish |
4:30-5 |
Lab Clean-up |