A14          

Review Questions,
M. J. Malachowski, Ph.D

Microscope Review



  1. Locate and learn the functions of the following parts of the microscope:
    • Supporting structures: base
      • arm
      • joint/hinge
      • stage
      • clip
      • tube
      • revolving nosepiece
    • Light regulating parts: Condenser
      • diaphragm and diaphragm leer
      • substage and substage adjustment
    • Magnifying parts:
      • eyepiece (ocular)
      • objectives
    • Focusing parts:
      • coarse adjustment
      • fine adjustment

  2. What is the significance of the numbers followed by an "x" on the eyepiece and on each objective? Be able to calculate the magnification provided by any combination of lenses.
  3. For scanning, low, and high power, be able to estimate visually the working distance between the lens and the slide.
  4. What is the field? The specimen? The image?
  5. How does the position of the image seen through the eyepiece compare with the position of the specimen placed on the slide? When you move the slide and specimen, which way does the image move?
  6. As you increase magnification, what changes do you observe in the following:
    • Size of the image?
    • Sharpness of the image?
    • Size of the field?
    • Brightness of the field?
  7. You should be able to locate specimens (both visible and invisible to the naked eye) under the microscope. Be able to adjust light, power, and focus for optimum viewing.
  8. What is the maximum magnification obtainable with the light microscope? With the electron microscope? What is the major disadvantage of observations made with the electron microscope?
















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    Updated 8/18/04 Miko Dr. Malachowski @ CCSFt