Biology Department          

Pipette Lab, M. J. Malachowski, Ph.D

NAME __________________________________

Pipette Laboratory
MaterialsSolutions
4 empty bullet tubes4 tubes with different food colorings
P 10 micropipette.
P 200 micropipette.
2 boxes of pipette tips.

  1. Obtain four clean bullet tubes, and place your initials and the following labels on the caps: A, B, C, and D.
  2. Obtain tubes I, II, III, and IV, containing various food colorings.
  3. Measure and mix the coloried solutions into your empty tubes according to the table below. use the P10 micropipette for the tubes A and B, and the P200 for tubes C and D.
    Allow everyone in your group to practice using both micropipettes. Choreograph your actions with your partners; for example, while you are operating the micropipette, they can be handing you the next tube and opening and closing all of the caps on the tubes.
  4. Your lab partner should check the accuracy of your pipettting by setting the micropipette to the total volume expected for each tube and then attempting to withdraw its entire contents.
    Tilt the tube and position the pipette tip against the bottom (but do not press too hard). If your pipetting is accurate, there should not be any fluid left in the tube or any air bubbles in the pipette tip.
  5. Afterwards, dispose of all of the used tips and tubes in the waste beaker.
    .
    .
    Fill in the Red boxes.
Pipette Exercise
micropipette-- TUBE-- ---- I ---- II---- III---- IV Total volume Checked
P10... A ... 1.5 l ... 5.0 l .... 0 l ... 2.5 l ..
P10... B ... 0.5 l .... 0 l ... 1.0 l ... 3.0 l ..
P200... C ... 45 l ... 50 l ... 80 l .... 0 l ..
P200... D .... 0 l ... 60 l ... 50 l ... 75 l ..



Updated 1/23/01 Miko mmalacho@ccsf.cc.ca.us